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Literature summary extracted from

  • Dobson, C.J.; Allinson, S.L.
    The phosphatase activity of mammalian polynucleotide kinase takes precedence over its kinase activity in repair of single strand breaks (2006), Nucleic Acids Res., 34, 2230-2237.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.78 expressed in Escherichia coli Rosetta2(DE3)pLysS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.78 D171A complete loss of activity Homo sapiens
2.7.1.78 D173A complete loss of activity Homo sapiens
2.7.1.78 K378A complete loss of activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Homo sapiens ADP + 5'-phospho-DNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 PNK
-
 Homo sapiens
2.7.1.78 polynucleotide kinase the phosphatase activity of the purified enzyme is significantly more active than its kinase activity Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP
-
 Homo sapiens